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"More than 400 proteins were visualised on the gels, of which 90 were differentially expressed between the two castes. These spots were cut out of the gels and identified by mass spectrometry, searching against the Apis mellifera (honeybee) database of the NCBI. A total of 61 proteins were identified this way, 41 being up-regulated in the drone and 20 in the workers."
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